rabbit polyclonal antibody against human pink1 protein  (Novus Biologicals)

Journal: Neural Regeneration Research

Article Title: Stress increases MHC-I expression in dopaminergic neurons and induces autoimmune activation in Parkinson's disease

doi: 10.4103/1673-5374.313057

Figure Lengend Snippet: The sequences of PINK1 gene

Article Snippet: The following primary antibodies were used for western blotting: rabbit polyclonal antibody against human PINK1 protein (BC100-494; Novus Biologicals, Centennial, CO, USA) and mouse monoclonal antibody against human glyceraldehyde 3-phosphate dehydrogenase (GAPDH; ab8245; Abcam).

Techniques: Negative Control

Journal: Neural Regeneration Research

Article Title: Stress increases MHC-I expression in dopaminergic neurons and induces autoimmune activation in Parkinson's disease

doi: 10.4103/1673-5374.313057

Figure Lengend Snippet: PINK1 is related to MHC-I expression in SH-SY5Y cells. (A) Flow cytometric analysis of MHC-I expression in neuron membranes. (B) Quantification of the flow cytometric analysis results. Data are expressed as the mean ± SEM ( n = 6; one-way analysis of variance followed by Bonferroni post hoc test). * P < 0.05. (C) Western blot assay of PINK1 expression in SH-SY5Y cells that had been transfected with control or PINK1 siRNA. (D) Fluorescence images showing the expression of MHC-1 (green) in SH-SY5Y cells. Scale bar: 5 μm. The arrow indicates MHC-I protein presenting on the cell surface. MHC-I: Major histocompatibility complex class I; MPP + : 1-methyl-4-phenylpyridinium; PINK1: PTEN-induced kinase 1.

Article Snippet: The following primary antibodies were used for western blotting: rabbit polyclonal antibody against human PINK1 protein (BC100-494; Novus Biologicals, Centennial, CO, USA) and mouse monoclonal antibody against human glyceraldehyde 3-phosphate dehydrogenase (GAPDH; ab8245; Abcam).

Techniques: Expressing, Western Blot, Transfection, Control, Fluorescence, Immunopeptidomics